Difference between revisions of "Part:BBa K243017"

Revision as of 20:22, 21 October 2009

Strep-DigA-Split Linker-Fok_a

This part is the counter part to BBa_K243010, it inhabits the active cutting site of our universal endonuclease. We prefer this part because it has another combiantion of basic parts than His-FluA-Split-Fok_i, which allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication] The purification is made with a Streptavidin column and the linkage is done by digoxigenin binding.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 278
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
INCOMPATIBLE WITH RFC[1000]
Illegal SapI.rc site found at 1132

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K243017 short</partinfo>
-This part is the counter part to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010],it inhabits the active cutting site of our universal endonuclease.
+This part is the counter part to [https://parts.igem.org/wiki/index.php?title=Part:BBa_K243010 BBa_K243010], it inhabits the active cutting site of our universal endonuclease.
-We prefer this part because it has an other combiantion of basic parts than His-FluA-Split-Fok_i, that allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication]
+We prefer this part because it has another combiantion of basic parts than His-FluA-Split-Fok_i, which allows us another way of purification and linkage to the labeled oligos, which were hybridized with the target DNA.[explication]
-The purification is made with a Streptavidin column and the linkage is done by dioxigenin binding.
+The purification is made with a Streptavidin column and the linkage is done by digoxigenin binding.