Difference between revisions of "Part:BBa K5117010:Design"
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===Source===
 
===Source===
 
Bacterium HR29
 
Bacterium HR29
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===References===
 
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Xi X., Ni K., Hao H., Shang Y., Zhao B., Qian Z. (2021): Secretory expression in Bacillus subtilis and biochemical characterization of a highly thermostable polyethylene terephthalate hydrolase from bacterium HR29. Enzyme and microbial technology 143, 109715.
 

Revision as of 17:20, 28 September 2024


BhrPET


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 579
    Illegal AgeI site found at 624
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

removal of restriction sites which are used for RFC10 or TypeIIS standards, addition of a signal peptide SPaprE for secretion in Bacillus subtilis, addition of start codon (ATG) and stop codon (TAA)

Source

Bacterium HR29