Difference between revisions of "Part:BBa K5117025"

 
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<i>eglS</i> gene of <i>Bacillus subtilis</i>, excluding its native signal peptide for secretion, encoding an endoglucanase (EC 3.2.1.4)
 
<i>eglS</i> gene of <i>Bacillus subtilis</i>, excluding its native signal peptide for secretion, encoding an endoglucanase (EC 3.2.1.4)
  
−
Downstream of the coding sequence, a long flexible linker (L3) has been added encoding the amino acids GGGEAAAKGGG.
+
Downstream of the coding sequence, a rigid linker (L3) has been added encoding the amino acids GGGEAAAKGGG leading to the formation of an alpha helix.
  
  

Revision as of 15:47, 28 September 2024


BsEglS-L3

eglS gene of Bacillus subtilis, excluding its native signal peptide for secretion, encoding an endoglucanase (EC 3.2.1.4)

Downstream of the coding sequence, a rigid linker (L3) has been added encoding the amino acids GGGEAAAKGGG leading to the formation of an alpha helix.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 526
  • 1000
    COMPATIBLE WITH RFC[1000]