Difference between revisions of "Part:BBa K5453004"
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<partinfo>BBa_K5453004 parameters</partinfo> | <partinfo>BBa_K5453004 parameters</partinfo> | ||
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| + | Subsequently, the plasmids R6k-dcas9-pfkA-zwf-M and pYB1c-Gatz-PGP-PGI were co-transformed into BW25113. Ninety-five colonies were randomly selected for fermentation experiments using glucose as the substrate, and their growth and production yields were screened. | ||
Revision as of 13:30, 28 September 2024
dcas9-sgRNA-pfkA-lac operator-lacI promoter-sgRNA-zwf-lac operator-lacI promoter
dcas9-sgRNA-pfkA-lac operator-lacI promoter-sgRNA1-zwf-lac operator-lacI promoter
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 1096
- 21INCOMPATIBLE WITH RFC[21]Illegal BamHI site found at 3375
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]
Subsequently, the plasmids R6k-dcas9-pfkA-zwf-M and pYB1c-Gatz-PGP-PGI were co-transformed into BW25113. Ninety-five colonies were randomly selected for fermentation experiments using glucose as the substrate, and their growth and production yields were screened.