Difference between revisions of "Part:BBa K5392015"
| Line 11: | Line 11: | ||
</style><div id="a1"> | </style><div id="a1"> | ||
| − | <img src="https://static.igem.wiki/teams/5392/pet28a-zatdt-page.png" width="407" height="262"/> | + | <img src="https://static.igem.wiki/teams/5392/pet28a-zatdt-page-1.png" width="407" height="262"/> |
</div></html> | </div></html> | ||
Revision as of 10:04, 28 September 2024
Vector-ZaTdT-KanR
Description
We incorporated the sequences of the ZaTdT gene into the pET28a vector. Then the vector plasmid was transfected into E.coli DH5-alpha competent cells for purification and amplification. In this way, plasmids with the ZaTdT gene were obtained.
Experiment
SDS-PAGE of ZaTdT
We transfected the ZaTdT gene-bonded pET28a plasmid into E.coli BL21(DE3) competent cell. After overnight, Colonies were then picked and performs protein expression. We identified the ZaTdT expression by SDS-PAGE and tested the incorporation of modified nucleotides by purified ZaTdT with polyacrylamide gel electrophoresis.
