Difference between revisions of "Part:BBa K175046"

Revision as of 19:48, 21 October 2009

Plasmid 1 for Transcriptional Cascade subproject TU Delft iGEM 09

This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here]. And the characterization of the delay device can be seen [http://2009.igem.org/Team:TUDelft/Delay_Results_Continue here].

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1436
Illegal AgeI site found at 1548
1000
COMPATIBLE WITH RFC[1000]

@@ Line 2: / Line 2: @@
 <partinfo>BBa_K175046 short</partinfo>
-This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here].
+This part is the plasmid 1 of the transcriptional cascade subproject of TU Delft iGEM. It generates the transcriptional regulator cI and RFP under the control of PTet promoter. This part in combination with the plasmid 2 (K175047) will lead to a delay in the expression of GFP after IPTG induction. The detailed description of the behavior and characterization of this part can be seen [http://2009.igem.org/Team:TUDelft/Results here]. And the characterization of the delay device can be seen [http://2009.igem.org/Team:TUDelft/Delay_Results_Continue here].
 <!-- Add more about the biology of this part here