Difference between revisions of "Part:BBa K243006"

(→‎Usage and Biology)
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<partinfo>BBa_K243006 short</partinfo>
 
<partinfo>BBa_K243006 short</partinfo>
  
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This part is a linker, it can be used to connect two parts and add additional space between these parts. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.
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This part is a linker, it can be used to connect two parts and add additional space between them. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.
  
  
 
===Usage and Biology===
 
===Usage and Biology===
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Linker were used normally to connect two peptides, it is important for that connection,that the linker itself has no influence of the connected peptides. So the sequence of the linker is designed for amino acids which not interact with the environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.<br>
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Linkers normally were used to connect two peptides. It is important for that connection, that the linker itself has no influence on the connected peptides. Hence the sequence of the linker is designed for amino acids which do not interact with their environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.<br>
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The length of the linker is important to guarantee the independent function of two connected parts. When the linker is too short there might be a sterical interference between the parts and when it is too long, it can cause an instability of the construct.Also it is important that the linker has a certain flexibility. <br>   
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The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short there might be a sterical interference between the parts and if it is too long, it can cause an instability of the construct. Also, it is important that the linker has a certain flexibility. <br>   
 
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<span class='h3bb'>'''Sequence and Features'''</span>
 
<span class='h3bb'>'''Sequence and Features'''</span>

Revision as of 19:48, 21 October 2009

Long Linker (Gly-Gly-Ser-Gly)x3

This part is a linker, it can be used to connect two parts and add additional space between them. That can be necessary to avoid interactions between these parts. We used this part to connect our protein domains with our lipocalin domains, for our project the universal endonuclease.The sequence produced the aminoacids Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly-Gly-Gly-Ser-Gly.


Usage and Biology

Linkers normally were used to connect two peptides. It is important for that connection, that the linker itself has no influence on the connected peptides. Hence the sequence of the linker is designed for amino acids which do not interact with their environment. The amino acids glycine and serine are zwitterionic and hydrophile, these properties make them a good choice for the repetitive sequence of the linker.
The length of the linker is important to guarantee the independent function of two connected parts. If the linker is too short there might be a sterical interference between the parts and if it is too long, it can cause an instability of the construct. Also, it is important that the linker has a certain flexibility.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]