Difference between revisions of "Part:BBa K245051:Design"
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===Source=== | ===Source=== | ||
| − | + | Composed from GyrB-SG and CutA1 basic parts according to assembly standard 37. | |
===References=== | ===References=== | ||
Latest revision as of 19:47, 21 October 2009
GyrB-SG-SG-CutA1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 428
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal AgeI site found at 100
Illegal AgeI site found at 238 - 1000COMPATIBLE WITH RFC[1000]
Design Notes
The part is inserted in BB-NIC-II-HisN vector (BBa_K245005) and can be assembled with other parts according to assembly standard 37. Composite parts were assembled (iGEM 2009, team Slovenia) by cutting front inserts with EcoRI and BspEI and front vectors with EcoRI and NgoMIV, thus forming RFC 37 Scar Sequence (BBa_K245009) beween separate parts.
Source
Composed from GyrB-SG and CutA1 basic parts according to assembly standard 37.