Difference between revisions of "Part:BBa K5108009:Design"

 
 
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<partinfo>BBa_K5108009 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5108009 SequenceAndFeatures</partinfo>
  
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===Design Notes===
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<h2 style="color: blue;">Design Notes</h2>
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<p>All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of basic parts was performed following In-Fusion Assembly (Takara, France).</p>
  
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<h2 style="color: blue;">Sources</h2>
  
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===Source===
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<p>Since this composite part consists of several BioBrick parts, several sources exist:
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<ul>
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<li><i>creA</i> and <i>crnA</i>: synthetized based on sequence from genome of <i>Pseudomonas putida</i>, codon optimized for <i>P. putida</i> with Integrated DNA Technologies <a href="https://eu.idtdna.com/pages/tools/codon-optimization-tool?returnurl=%2FCodonOpt" target="blank">codon optimizer tool.</a> This step permits to lower complexity and minimize secondary structures.</li>
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<li><i>aprA CHA0</i> and <i>hcnA CHA0</i> RBS: synthetized based on sequence from genome of <i>Pseudomonas protegens</i>.</li>
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<li><i>Ptet</i> promoter: amplified from the pHD_SS9_PLtetO1_TLt0_eutC-GFP plasmid provided by our supervisor Denis Jallet (Toulouse Biotechnology Institute, France).</li>
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<li>pSEVA438 plasmid: provided by <a href="https://seva-plasmids.com" target="blank">seva-plasmids.</a>
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===References===
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Latest revision as of 08:49, 28 September 2024


RBS aprA CHA0 - creA - RBS hcnA CHA0 - crnA


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1329
    Illegal NgoMIV site found at 1931
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 249
    Illegal BsaI site found at 1036
    Illegal BsaI site found at 1467
    Illegal BsaI.rc site found at 1815

Design Notes

All BioBrick parts used for assembling the composite part are compatible with the RFC10 Biobrick standard. Fusion of basic parts was performed following In-Fusion Assembly (Takara, France).

Sources

Since this composite part consists of several BioBrick parts, several sources exist:

  • creA and crnA: synthetized based on sequence from genome of Pseudomonas putida, codon optimized for P. putida with Integrated DNA Technologies codon optimizer tool. This step permits to lower complexity and minimize secondary structures.
  • aprA CHA0 and hcnA CHA0 RBS: synthetized based on sequence from genome of Pseudomonas protegens.
  • Ptet promoter: amplified from the pHD_SS9_PLtetO1_TLt0_eutC-GFP plasmid provided by our supervisor Denis Jallet (Toulouse Biotechnology Institute, France).
  • pSEVA438 plasmid: provided by seva-plasmids.