Difference between revisions of "Part:BBa K185033"
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It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added. | It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added. | ||
| − | And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031. | + | And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031. This part improve on the basis of lac QPI system([[Part:BBa_K098986|BBa_K098986]]) |
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Revision as of 19:45, 21 October 2009
An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31
It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added. And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031. This part improve on the basis of lac QPI system(BBa_K098986)
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NheI site found at 7
Illegal NheI site found at 30 - 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]