Difference between revisions of "Part:BBa K185033"

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It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added.  
 
It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added.  
And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031.
+
And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031. This part improve on the basis of lac QPI system([[Part:BBa_K098986|BBa_K098986]])
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 19:45, 21 October 2009

An inverter of a special lactose operon system based on J23110-rbs34-lacI-dter-plac-rbs31

It is an inverter which its downsteam gene will always be repressed by lacI protein unless IPTG is added. And the synthetic rate of the downsteam gene is low because the low binding efficiency of B0031. This part improve on the basis of lac QPI system(BBa_K098986)

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]