Difference between revisions of "Part:BBa K079031:Experience"

Revision as of 19:39, 21 October 2009

Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software. The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Table 1). The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.1 and Fig.2, respectively). Fluorescence was then normalized on the OD value (Fig.4).

Table 1 - Promoter fluorescence ratio after microscope analysys

Fig.1 - Growth curve

Fig.2 - Fluorescence

Fig.3 - Fluorescence curve over OD

UNIQdea28336d0637553-partinfo-00000000-QINU UNIQdea28336d0637553-partinfo-00000001-QINU

@@ Line 4: / Line 4: @@
 Dh5alpha cells transformed with BBa_K079032 and BBa_K079031 were inoculated
 in M9 medium at 8.00 p.m. after O/N growth (about 12 h), samples were collected and slide prepared for image acquisition with the VIFluoR software.
-The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Fig.1).
+The BBa_K079032/ BBa_K079031 fluorescence ratio was equal to 1.20±0.4 (Table 1).
-The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.2 and Fig.3, respectively). Fluorescence was then normalized on the OD value (Fig.4).
+The same samples were diluited to an OD equal 0.1 and a growth in time was performed with a Tekcan spectrofluorimeter. Both optical density (OD) and fluorescence level were analized for 12 h (Fig.1 and Fig.2, respectively). Fluorescence was then normalized on the OD value (Fig.4).
-[[Image:TabellaPromotori3.png|center|800px |thumb|Fig.1 - Table of promoter]]
+[[Image:TabellaPromotori3.png|center|800px |thumb|Table 1 - Promoter fluorescence ratio after microscope analysys]]
-[[Image:GrowthCurve1.png|center|800px |thumb|Fig.2 - Growth curve]]
+[[Image:GrowthCurve1.png|center|800px |thumb|Fig.1 - Growth curve]]
-[[Image:FluorescenceCurveAbsolute1.png|center|800px |thumb|Fig.3 - Fluorescence]]
+[[Image:FluorescenceCurveAbsolute1.png|center|800px |thumb|Fig.2 - Fluorescence]]
-[[Image:FluorescenceCurveOverOD1.png|center|800px |thumb|Fig.4 - Fluorescence curve over OD]]
+[[Image:FluorescenceCurveOverOD1.png|center|800px |thumb|Fig.3 - Fluorescence curve over OD]]
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