Difference between revisions of "Part:BBa K5468003"

Latest revision as of 03:56, 28 September 2024

secA

SecA is a key component of the Sec translocation pathway in bacteria, responsible for driving the transport of proteins from the cytoplasm to the cell membrane or extracellular environment. It functions as an ATPase, providing the energy needed to push preproteins through the SecYEG translocon. In systems like Bacillus subtilis, SecA plays a critical role in exporting proteins such as enzymes and biosurfactants, including surfactin. Overexpression of SecA can enhance the secretion efficiency of these proteins, leading to higher yields in biotechnological applications like surfactin production.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 1282
1000
INCOMPATIBLE WITH RFC[1000]
Illegal BsaI site found at 1719
Illegal SapI.rc site found at 2098

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 <partinfo>BBa_K5468003 short</partinfo>
-The SecA protein encoded by the secA gene is a core component of the Sec secretion pathway (also known as the general secretion pathway or Sec pathway). The Sec secretion pathway is one of the primary protein secretion pathways in bacteria, responsible for transporting a large number of proteins from the cytoplasm to the extracellular space or onto organelle membranes. As both the binding subunit of the translocase and an ATPase, the SecA protein harnesses the energy generated from ATP hydrolysis to drive the translocation of proteins through the SecYEG channel in the cell membrane. Specifically, the SecA protein interacts with the SecYEG complex to form a dynamic translocation machinery. Through its ATPase activity, the SecA protein continuously changes its conformation, thereby propelling protein molecules across the cell membrane.
+SecA is a key component of the Sec translocation pathway in bacteria, responsible for driving the transport of proteins from the cytoplasm to the cell membrane or extracellular environment. It functions as an ATPase, providing the energy needed to push preproteins through the SecYEG translocon. In systems like Bacillus subtilis, SecA plays a critical role in exporting proteins such as enzymes and biosurfactants, including surfactin. Overexpression of SecA can enhance the secretion efficiency of these proteins, leading to higher yields in biotechnological applications like surfactin production.
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