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| | + | A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities. |
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| − | __NOTOC__
| + | A hexahistidine tag for nickel column purification was added to A0A419DFF0. |
| − | <partinfo>BBa_K5035004 short</partinfo>
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| − | <partinfo>BBa_K5035004 SequenceAndFeatures</partinfo>
| + | References: |
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| − | | + | Momper, Lily et al. “Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community.” The ISME journal vol. 11,10 (2017): 2319-2333. doi:10.1038/ismej.2017.94 |
| − | ===Design Notes===
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| − | A hexahistidine tag for nickel column purification was added to the A0A419DFF0 sequence.
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| − | ===Source===
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| − | ATGCATCATCATCATCATCATTCTAAATACCATAGCATTCTGTCCCGCCGCGAATTCCTGAAAGCACTGGGCATGGGTTCTGCCGGCCTGGGTGCACTGGCAGTCGCACCACCTGCGATCCGTGACATGGACGAAGTAATCGCGAGCCCTCAAGCAGACTGGAAACGTCCGGCTTGGGTTAAAGAGGTTGACAAACCGACTGTAGAAATCGACTGGAAAATCCTGGAACGTTTTGATTACCGCGAGGTAATGTTCGTCAACGGTTTCACCAAAGCGGTTGGCAAAGAGACTGCTGGCATCCTGTCTCAGGTGGGCCTGGCGAACGCGAAAAAATGGATCCGCGATAAACGCCCGGGTCACACCCTGAAAGACTACGCTGTTAAGATGGGTGCGCAAGGCTACGCGTTTGACCCGCACTCCTACCTGGGCTACAAAAAATCTCCGACGCCGGAATCTCTGGGTGTGCCACGCTGGGAAGGTACTCCGGAGGAGAACGCGCGCATGGTTCGTACCGTCATGCGTCTGTATGGCGCAGTAGATGTGGCTTATGTTGAACTGGACACCGACACCACTGAAAAACTGATTTACACCTACGATGTCGATGGCAAACAAATGGAAATTAAAGACGTTGAGCTGGGCGAAGAAAGCGAAAAAGTTAAAATCCTGCCGAAGAAAGCCCGCTGGGTGATCGTATACACTATGATGATGTCCCACGAACTGACCCGCCGTCTGCCGTCCTTCAACGCTGAAGCTACCGTATACATGCCGTACGCTCAGGGTCCATGGCTGCAGGATCGTTTTCAGGATTTCATCCGCACCCTGGGTTACAACTGCTACGGTGAACCGCGTCCAAACGCACTGGGTACGTCCGTCGGCCTGGGTGTTATGGGCGGTCTGGGCGAAATCTCCCGCATCGAACACATCATTACGCCGGGTCGTGGCTTTTCTCACCGCGTTTTTAAGATGATCACGGATCTGCCGCTGGCACCGACCAAACCGATCGATACTGGTGTTATGGATTTCTGTCGTGTCTGCAAGAAATGCTCCGACATGTGCCCGGCGCACGCAATTGCGCCGGATACCGAACCGACTTGGAAAATCCCGGGTCCTTATAAAAACCCGGGTGTTAAAGGTTGGTTCCGCATTGAGCCTCTGTGCTATACGTATTGGCGTCAAACGGGTACGGGTTGCGGCTTTTGTCTGGCCCTGTGTCCGCTGAACCGTCCGCAGAACACGTCTTACTTCAAAACGATGCGCTCTACCATCGCTAAAACCACTGCCCTGAACCGCACTTTCCGCAAAATGGATGACCTGCTGGATTGGGGCCCACGCCGTGATCCAGCAGGCTTCTGGGACCTGGAAATGCCATCTTTTGGTTGGGATCTACTCTGGCGTCGATGA
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| − | ===References===
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Revision as of 03:21, 28 September 2024
A0A419DFF0 is a corrinoid iron-sulfur-containing reductive dehalogenase produced by the Dehalococcoidia bacterium. A0A419DFF0 is structurally similar to enzyme A6RdhA, a corrinoid iron-sulfur-containing reductive dehalogenase capable of degrading PFOA. Because of A0A419DFF0's structural and familial similarity to A6RdhA, it is predicted to have similar PFOA degrading abilities.
A hexahistidine tag for nickel column purification was added to A0A419DFF0.
References:
Momper, Lily et al. “Energy and carbon metabolisms in a deep terrestrial subsurface fluid microbial community.” The ISME journal vol. 11,10 (2017): 2319-2333. doi:10.1038/ismej.2017.94
