Difference between revisions of "Part:BBa K5136035"

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==Biology==
 
==Biology==
  
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==INPNC==
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===INPNC===
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<br>Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).  
+
Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).
 +
 
 
==SpyCatcher==
 
==SpyCatcher==
 
<br>SpyCatcher is a engineered split fragment of the fibronectin-binding protein (FbaB) in Streptococcus pyogenes, containing 116 residues. SpyTag is a peptide tag of 13 residues developed by previous researchers. SpyCatcher could steadily bind to SpyTag by forming an isopeptide bond (2).
 
<br>SpyCatcher is a engineered split fragment of the fibronectin-binding protein (FbaB) in Streptococcus pyogenes, containing 116 residues. SpyTag is a peptide tag of 13 residues developed by previous researchers. SpyCatcher could steadily bind to SpyTag by forming an isopeptide bond (2).

Revision as of 03:06, 28 September 2024


inpnc-His tag-SpyCatcher

Biology

INPNC

Ice nucleoprotein (INP), an outer membrane protein from Pseudomonas syringae, has been used as a surface anchor in many researches. The truncated version of INP, namely INPNC which contains only the N- and C-terminal portion of INP, has excellent capacity to anchor target proteins on the cell membrane (1).

SpyCatcher


SpyCatcher is a engineered split fragment of the fibronectin-binding protein (FbaB) in Streptococcus pyogenes, containing 116 residues. SpyTag is a peptide tag of 13 residues developed by previous researchers. SpyCatcher could steadily bind to SpyTag by forming an isopeptide bond (2).

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 481
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 330
    Illegal XhoI site found at 1089
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 72
    Illegal NgoMIV site found at 405
    Illegal AgeI site found at 429
  • 1000
    COMPATIBLE WITH RFC[1000]