Difference between revisions of "Part:BBa K5115083"
 
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===Characterization===
 
===Characterization===
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====Agarose gel electrophoresis====
 
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{|
 
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| <html><img style="width:400px" src="https://static.igem.wiki/teams/5115/registry/joe-rcna-donor.png" alt="contributed by Fudan iGEM 2024"></html>
 
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
 
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M: DNA Marker; Lanes 1-5(RcnA KO doner): Successful construction of the RcnA KO donor (657 bp) in the JOE plasmid; Lane 6: Negative control (NC) using original pJOE_pvdJ plasmid (approximately 2000 bp).
 
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'''
 
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|}
 
  
 
===Sequence and Features===
 
===Sequence and Features===

Latest revision as of 17:38, 27 September 2024


deleted083

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Sequence and Features

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References