Difference between revisions of "Part:BBa K5115083"

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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
M: DNA Marker; Lanes 1-5(RcnA KO doner): Successful construction of the RcnA KO donor (657 bp) in the JOE plasmid; Lane 6: Negative control (nc) using original pJOE_pvdJ plasmid (approximately 2000 bp).
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M: DNA Marker; Lanes 1-5(RcnA KO doner): Successful construction of the RcnA KO donor (657 bp) in the JOE plasmid; Lane 6: Negative control (NC) using original pJOE_pvdJ plasmid (approximately 2000 bp).
 
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====Successful Protein Expression====
 
  
  

Revision as of 17:02, 27 September 2024


deleted083

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2024
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

M: DNA Marker; Lanes 1-5(RcnA KO doner): Successful construction of the RcnA KO donor (657 bp) in the JOE plasmid; Lane 6: Negative control (NC) using original pJOE_pvdJ plasmid (approximately 2000 bp).



Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]



References