Difference between revisions of "Part:BBa K5115083"
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====Agarose gel electrophoresis==== | ====Agarose gel electrophoresis==== | ||
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| − | | <html><img style="width: | + | | <html><img style="width:400px" src="https://static.igem.wiki/teams/5115/registry/joe-rcna-donor.png" alt="contributed by Fudan iGEM 2024"></html> |
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| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | | '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures. | ||
Revision as of 17:00, 27 September 2024
deleted083
Contents
Introduction
Usage and Biology
Characterization
Agarose gel electrophoresis
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| Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
M: DNA Marker; Lanes 1-5(RcnA KO doner): Successful construction of the RcnA KO donor (657 bp) in the JOE plasmid; Lane 6: Negative control (nc) using original pJOE_pvdJ plasmid (approximately 2000 bp). |
Successful Protein Expression
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal BglII site found at 12
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]