Difference between revisions of "Part:BBa K5115083"

(Agarose gel electrophoresis)
Line 12: Line 12:
 
====Agarose gel electrophoresis====
 
====Agarose gel electrophoresis====
 
{|
 
{|
| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/gal-pgm-mscarlet.png" alt="contributed by Fudan iGEM 2023"></html>
+
| <html><img style="width:200px" src="https://static.igem.wiki/teams/5115/registry/joe-rcna-donor.png" alt="contributed by Fudan iGEM 2024"></html>
 
|-
 
|-
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
 
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.  
From left lane(1) to right lane(3) indicate the successful construction of ''galU'', ''galU'' + ''pgmA'', and ''galU'' + ''pgmA'' + mScarlet. '''
+
M: DNA Marker; From left lane(1) to right lane(5) indicate the successful construction of ''galU'', ''galU'' + ''pgmA'', and ''galU'' + ''pgmA'' + mScarlet. '''
  
 
|}
 
|}
 +
 
====Successful Protein Expression====
 
====Successful Protein Expression====
 
{|
 
{|

Revision as of 16:40, 27 September 2024


deleted083

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2024
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

M: DNA Marker; From left lane(1) to right lane(5) indicate the successful construction of galU, galU + pgmA, and galU + pgmA + mScarlet.

Successful Protein Expression

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

contributed by Fudan iGEM 2023