Difference between revisions of "Part:BBa K5115083"

Line 10: Line 10:
  
 
===Characterization===
 
===Characterization===
 +
====Agarose gel electrophoresis====
 +
{|
 +
| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/dna-gel/gal-pgm-mscarlet.png" alt="contributed by Fudan iGEM 2023"></html>
 +
|-
 +
| '''Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.
 +
From left lane(1) to right lane(3) indicate the successful construction of ''galU'', ''galU'' + ''pgmA'', and ''galU'' + ''pgmA'' + mScarlet. '''
  
 +
|}
 +
====Successful Protein Expression====
 +
{|
 +
| <html><img style="width:200px" src="https://static.igem.wiki/teams/4765/wiki/zsl/protein-gel/gp-1-pg-1-1.png" alt="contributed by Fudan iGEM 2023"></html>
 +
|-
  
 
<!-- -->
 
<!-- -->

Revision as of 16:25, 27 September 2024


deleted083

contributed by Fudan iGEM 2023

Introduction

Usage and Biology

Characterization

Agarose gel electrophoresis

contributed by Fudan iGEM 2023
Figure 1. Agarose gel electrophoresis of PCR products, amplified from bacterial colonies/cultures.

From left lane(1) to right lane(3) indicate the successful construction of galU, galU + pgmA, and galU + pgmA + mScarlet.

Successful Protein Expression

Sequence and Features

Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 12
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

contributed by Fudan iGEM 2023