Difference between revisions of "Part:BBa K5143026"
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| − | The Alphafactor-venus composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor. | + | The Alphafactor-venus composite part was used in a larger composite part <a href="https://parts.igem.org/Part:BBa_K5143027" target="_blank">BBa_K5143027</a>that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor. |
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Revision as of 15:17, 27 September 2024
Alphafactor-Venus, a secreted chromoprotein
Description
The Alphafactor-venus composite part was used in a larger composite part BBa_K5143027that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.
Construction
Alphafactor-Venus is composed of the alphafactor V1 BBa_K5143009 and the venus chromoprotein BBa_K5143015.
Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (BBa_K4081994).
References
1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011).
2) https://pubmed.ncbi.nlm.nih.gov/25871405
3) https://parts.igem.org/Part:BBa_K5143015
Sequence and Features
- 10INCOMPATIBLE WITH RFC[10]Unknown
- 12INCOMPATIBLE WITH RFC[12]Unknown
- 21INCOMPATIBLE WITH RFC[21]Unknown
- 23INCOMPATIBLE WITH RFC[23]Unknown
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]