Difference between revisions of "Part:BBa K5143026"
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<h1>Construction</h1> | <h1>Construction</h1> | ||
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| + | Alphafactor-Venus is composed of the alphafactor V1 <a href="https://parts.igem.org/Part:BBa_K5143009" target="_blank">BBa_K5143009</a> and the venus chromoprotein <a href="https://parts.igem.org/Part:BBa_K5143015" target="_blank">BBa_K5143015</a>. <br> | ||
Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (<a href="https://parts.igem.org/Part:BBa_K4081994" target="_blank">BBa_K4081994</a>). | Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (<a href="https://parts.igem.org/Part:BBa_K4081994" target="_blank">BBa_K4081994</a>). | ||
</p> | </p> | ||
Revision as of 15:10, 27 September 2024
Alphafactor-Venus, a secreted chromoprotein
Description
The Alphafactor-venus composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.
Construction
Alphafactor-Venus is composed of the alphafactor V1 BBa_K5143009 and the venus chromoprotein BBa_K5143015.
Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (BBa_K4081994).
References
1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011).
2) https://pubmed.ncbi.nlm.nih.gov/25871405
3) https://parts.igem.org/Part:BBa_K5143015
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]