Difference between revisions of "Part:BBa K5143026"

 
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This composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor.
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    <h1>Description</h1>
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        The Alphafactor-venus composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor
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    <h1>Construction</h1>
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      Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in <i>Saccharomyces cerevisiae</i>.  In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (<a href="https://parts.igem.org/Part:BBa_K4081994" target="_blank">BBa_K4081994</a>).
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    <h1>References</h1>
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        1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011). <br>
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2) https://pubmed.ncbi.nlm.nih.gov/25871405 <br>
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3) https://parts.igem.org/Part:BBa_K5143015 <br>
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<span class='h3bb'>Sequence and Features</span>
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<h1>Sequence and Features</h1>
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<partinfo>BBa_K5143026 SequenceAndFeatures</partinfo>
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<partinfo>BBa_K5143007 SequenceAndFeatures</partinfo>
  
  
 
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===Functional Parameters===
 
===Functional Parameters===
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<partinfo>BBa_K5143007 parameters</partinfo>
 
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Revision as of 14:36, 27 September 2024


Alphafactor-Venus, a secreted chromoprotein

Protein Description

Description

The Alphafactor-venus composite part was used in a larger composite part that enables our team to test the transcriptional activity of GAP promoter and to test the secretion of the protein by the alphafactor

Construction

Alphafactor-Venus was synthesised and its nucleotide sequence optimised for synthesis and expression in Saccharomyces cerevisiae. In our construction, Alphafactor-Venus is under the control of gap promoter to test its activation (BBa_K4081994).

References

1) 1. Qin, X. et al. GAP Promoter Library for Fine-Tuning of Gene Expression in Pichia pastoris. Appl Environ Microbiol 77, 3600–3608 (2011).
2) https://pubmed.ncbi.nlm.nih.gov/25871405
3) https://parts.igem.org/Part:BBa_K5143015


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BamHI site found at 256
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 226
    Illegal AgeI site found at 265
  • 1000
    COMPATIBLE WITH RFC[1000]