Difference between revisions of "Part:BBa K5291037:Design"

(→‎Design Notes)
(→‎Design Notes)
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===Design Notes===
 
===Design Notes===
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We replace the promoter of <i>sok</i> by P<sup>opdH</sup>, a promoter able to sense the concentration of citrate, and replace the promoter of <i>hok</i>/<i>mok</i> by PcW, a common weak constitutive promoter in <i>Pseudomonas aeruginosa</i>. When the bacteria are in the mangrovem, the relatively high concentration of citrate is able to activate P<sup>opdH</sup> and <i>sok</i> can be expressed to antagonize the effect of <i>hok</i>/<i>mok</i> due to the weak function of PcW. When the bacteria are out of the mangroves, P<sup>opdH</sup> cannot come into effect and the expression of <i>sok</i> is terminated, which enables <i>hok</i> to kill the cells.<br>
+
We replace the promoter of <i>sok</i> by P<sub>opdH</sub>, a promoter able to sense the concentration of citrate, and replace the promoter of <i>hok</i>/<i>mok</i> by PcW, a common weak constitutive promoter in <i>Pseudomonas aeruginosa</i>. When the bacteria are in the mangrovem, the relatively high concentration of citrate is able to activate P<sub>opdH</sub> and <i>sok</i> can be expressed to antagonize the effect of <i>hok</i>/<i>mok</i> due to the weak function of PcW. When the bacteria are out of the mangroves, P<sub>opdH</sub> cannot come into effect and the expression of <i>sok</i> is terminated, which enables <i>hok</i> to kill the cells.<br>
 
When the plasmid with <i>hok</i>/<i>sok</i> system is lost, both of the genes cannot be expressed. However, the half-life of the <i>hok</i> mRNA is longer than the one of the <i>sok</i> antisense RNA, which leads to the result that the <i>hok</i> mRNA still exists when all the <i>sok</i> antisense RNA is degraded. The rest <i>hok</i> mRNA can encode toxin to kill the cell, achieving the function of plasmid anti-loss.
 
When the plasmid with <i>hok</i>/<i>sok</i> system is lost, both of the genes cannot be expressed. However, the half-life of the <i>hok</i> mRNA is longer than the one of the <i>sok</i> antisense RNA, which leads to the result that the <i>hok</i> mRNA still exists when all the <i>sok</i> antisense RNA is degraded. The rest <i>hok</i> mRNA can encode toxin to kill the cell, achieving the function of plasmid anti-loss.
  

Revision as of 13:30, 27 September 2024


pAB-hok/sok


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NotI site found at 146
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 16
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We replace the promoter of sok by PopdH, a promoter able to sense the concentration of citrate, and replace the promoter of hok/mok by PcW, a common weak constitutive promoter in Pseudomonas aeruginosa. When the bacteria are in the mangrovem, the relatively high concentration of citrate is able to activate PopdH and sok can be expressed to antagonize the effect of hok/mok due to the weak function of PcW. When the bacteria are out of the mangroves, PopdH cannot come into effect and the expression of sok is terminated, which enables hok to kill the cells.
When the plasmid with hok/sok system is lost, both of the genes cannot be expressed. However, the half-life of the hok mRNA is longer than the one of the sok antisense RNA, which leads to the result that the hok mRNA still exists when all the sok antisense RNA is degraded. The rest hok mRNA can encode toxin to kill the cell, achieving the function of plasmid anti-loss.

Source

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References