Difference between revisions of "Part:BBa K5143015"
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     <h1>Description</h1>
 
     <h1>Description</h1>
 
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         Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (Part:BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.
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         Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by <i>Aequorea victoria</i>. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (<a href="https://parts.igem.org/Part:BBa_K4081994">BBa_K4081994</a>). The excitation wavelength is 516nm and the emission wavelength is 530nm.
 
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     <h1>Construction</h1>
 
     <h1>Construction</h1>
 
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       The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (Part:BBa_K5143009) in 5'. See the composite part here:
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       The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in <I>Saccharomyces cerevisiae</i>. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (<a href="https://parts.igem.org/Part:BBa_K5143009">BBa_K5143009</a>) in 5'. See the composite part here:
 
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     <h1>References</h1>
 
     <h1>References</h1>
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<span class='h3bb'>Sequence and Features</span>
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<h1>Sequence and Features</h1>
 
<partinfo>BBa_K5143015 SequenceAndFeatures</partinfo>
 
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Revision as of 13:21, 27 September 2024


Venus : reporter Yellow Fluorescent Protein

Protein Description

Description

Venus is a Yellow Fluorescent Protein that was developed to overcome limitations to the use of Green Fluorescent Protein produced by Aequorea victoria. Therefore, Venus can be used as a reporter system. Here, Venus was used as a transcriptional reporter, to control the transcriptional efficiency of the yeast promoter pGAP (BBa_K4081994). The excitation wavelength is 516nm and the emission wavelength is 530nm.

Venus
Venus Yellow Fluorescent Protein used as a transcriptional reporter.

Construction

The Venus used was synthetized based on a sequence from a yeast toolkit so the sequence is optimized for expression in Saccharomyces cerevisiae. In our genetic construction, the gene Venus was synthetized in fusion with the secretion signal alpha-factor (BBa_K5143009) in 5'. See the composite part here:

References

1. Nagai, T. et al. A variant of yellow fluorescent protein with fast and efficient maturation for cell-biological applications. Nat Biotechnol 20, 87–90 (2002).
2. Lee, M. E., DeLoache, W. C., Cervantes, B. & Dueber, J. E. A Highly Characterized Yeast Toolkit for Modular, Multipart Assembly. ACS Synth. Biol. 4, 975–986 (2015).


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]