Difference between revisions of "Part:BBa K5034212:Design"

 
Line 13: Line 13:
 
===Source===
 
===Source===
  
−
We synthesized the fragment using a chemical synthesis method,.
+
Phosphate-AMP phosphotransferase(PAP) from <i>Acinetobacter johnsonii</i>.
  
 
===References===
 
===References===
 +
 +
Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004

Revision as of 11:22, 27 September 2024


PolyP + AMP --> ADP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We synthesized the fragment using a chemical synthesis method, with the aim of expressing the protein in Shewanella and regulating cellular phosphorus metabolism and electron transfer.


Source

Phosphate-AMP phosphotransferase(PAP) from Acinetobacter johnsonii.

References

Itoh, H., & Shiba, T. (2004). Polyphosphate synthetic activity of polyphosphate:AMP phosphotransferase in Acinetobacter johnsonii 210A. Journal of Bacteriology, 186(15), 5178-5181. doi:10.1128/jb.186.15.5178-5181.2004