Difference between revisions of "Part:BBa K5311016"
 
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<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
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Latest revision as of 10:14, 27 September 2024

MG_010 + RNA thermometer ForU + sfGFP + His-tag

Genetic construct created to produce sfGFP to induce fluorescence in C. acnes and E. coli. This expression is regulated with an RNA Thermometer that allows expression when the optimal temperature of 37°C is achieved

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 126
  • 1000
    COMPATIBLE WITH RFC[1000]