Difference between revisions of "Part:BBa K5299204:Design"

 
(→‎Design Notes)
Line 7: Line 7:
  
 
===Design Notes===
 
===Design Notes===
−
Designed using the Golden Braid method.
+
Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.
−
 
+
−
 
+
  
 
===Source===
 
===Source===

Revision as of 09:47, 27 September 2024


BBa_J45992 - BBa_B0030 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 228


Design Notes

Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.

Source

The promoter BBa_J45992 already existed in the Registry. The RBS BBa_B0030 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The terminator BBa_B0015 already existed in the Registry.

References