Difference between revisions of "Part:BBa K5351000"

 
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pSCm-N20
 
  
  
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===Usage and Biology===
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
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===Functional Parameters===
 
===Functional Parameters===
 
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<span id="origin"></span>
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= Origin =
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*E. coli*; synthesized
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<span id="properties"></span>
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= Properties =
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The plasmid pSCm-N20 is a genetic construct containing specific DNA sequences that enable the expression of a particular gene or genes in a host organism. It includes a promoter region for gene expression and possibly other regulatory elements necessary for the function of the inserted gene. It is used as the vector plasmid in our research.
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= Experimental Approach =
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5351/bba-k5351000/1.png" width="50%" style="display:block; margin:auto;" alt="Plasmid map of pSCm-N20" >
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    <div style="text-align:center;">
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        <caption>Figure 1. Plasmid map of pSCm-N20.</caption>
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The plasmid pSCm was digested to obtain a linear backbone with a length of 5984 bp.
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<div style="text-align:center;">
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    <img src="https://static.igem.wiki/teams/5351/bba-k5351000/2.png" width="50%" style="display:block; margin:auto;" alt="The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone" >
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    <div style="text-align:center;">
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        <caption>Figure 2. The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone.</caption>
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The band shown in Figure 2 indicates that the electrophoresis results are consistent with the expected plasmid size, confirming successful linearization of the plasmid backbone.

Revision as of 09:16, 27 September 2024

pSCm-N20



Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 12
    INCOMPATIBLE WITH RFC[12]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal NheI site found at 1135
    Illegal NheI site found at 4384
    Illegal NotI site found at 2401
  • 21
    INCOMPATIBLE WITH RFC[21]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
    Illegal BamHI site found at 2246
    Illegal BamHI site found at 2357
    Illegal XhoI site found at 2408
  • 23
    INCOMPATIBLE WITH RFC[23]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 25
    INCOMPATIBLE WITH RFC[25]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Plasmid lacks a prefix.
    Plasmid lacks a suffix.


Origin

  • E. coli*; synthesized

Properties

The plasmid pSCm-N20 is a genetic construct containing specific DNA sequences that enable the expression of a particular gene or genes in a host organism. It includes a promoter region for gene expression and possibly other regulatory elements necessary for the function of the inserted gene. It is used as the vector plasmid in our research.

Experimental Approach

Plasmid map of pSCm-N20
Figure 1. Plasmid map of pSCm-N20.

The plasmid pSCm was digested to obtain a linear backbone with a length of 5984 bp.

The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone
Figure 2. The gel electrophoresis validation of the linear pSCm-N20 plasmid backbone.

The band shown in Figure 2 indicates that the electrophoresis results are consistent with the expected plasmid size, confirming successful linearization of the plasmid backbone.