Difference between revisions of "Part:BBa K5299202:Design"

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===References===
 
===References===
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*[1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. <i> Plasmid, 109 </i>(102491), 102491. doi:10.1016/j.plasmid.2020.102491
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*[2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. <i>  Nature Biotechnology, 24 </i>(1), 79–88. doi:10.1038/nbt1172

Latest revision as of 09:13, 27 September 2024


BBa_J23119 - BBa_B0030 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 64


Design Notes

Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.

Source

The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0030 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The BBa_B0015 already existed in the Registry.

References

  • [1]: Jaishankar, J., & Srivastava, P. (2020). Strong synthetic stationary phase promoter-based gene expression system for Escherichia coli. Plasmid, 109 (102491), 102491. doi:10.1016/j.plasmid.2020.102491
  • [2]: Pédelacq, J.-D., Cabantous, S., Tran, T., Terwilliger, T. C., & Waldo, G. S. (2006). Engineering and characterization of a superfolder green fluorescent protein. Nature Biotechnology, 24 (1), 79–88. doi:10.1038/nbt1172