Difference between revisions of "Part:BBa K5299202:Design"

 
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===Design Notes===
 
===Design Notes===
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Designed using the Golden Braid cloning method.  
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Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.
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===Source===
 
===Source===

Revision as of 09:12, 27 September 2024


BBa_J23119 - BBa_B0030 - BBa_I746916 - BBa_B0015


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 64


Design Notes

Designed using the Golden Braid assembly method, that utilises type IIS enzymes. We used BsmBI to create Level 0 constructs, and BsaI for Level 1 constructs.

Source

The promoter BBa_J23119 already existed in the Registry. The RBS BBa_B0030 already existed in the Registry. The sfGFP BBa_I746916 already existed in the Registry. The BBa_B0015 already existed in the Registry.

References