Difference between revisions of "Part:BBa K5299120"
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The AAC (NW_009276936.1 VDAG_07535) gene codes for an ADP/ATP carrier protein that helps generate energy for V. dahliae. | The AAC (NW_009276936.1 VDAG_07535) gene codes for an ADP/ATP carrier protein that helps generate energy for V. dahliae. | ||
It is essential for maintaining the ADP/ATP balance by mediating the exchange of ADP and ATP between the mitochondria and the cytoplasm. [1] | It is essential for maintaining the ADP/ATP balance by mediating the exchange of ADP and ATP between the mitochondria and the cytoplasm. [1] | ||
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| + | <img class="image" src='https://static.igem.wiki/teams/5299/myrto/wet-lab-33.png' width="700" height="600"> | ||
| + | <figcaption><center><b>Figure 1: ADP/ ATP carrier transports ATP out of the mitochondria into the cytoplasm and brings ADP back in.</b></center></figcaption> | ||
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<h1>Use</h1> | <h1>Use</h1> | ||
This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [2].<br> | This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [2].<br> | ||
Latest revision as of 09:05, 27 September 2024
AAC protein (V. dahliae)
Biology
The AAC (NW_009276936.1 VDAG_07535) gene codes for an ADP/ATP carrier protein that helps generate energy for V. dahliae. It is essential for maintaining the ADP/ATP balance by mediating the exchange of ADP and ATP between the mitochondria and the cytoplasm. [1]
Use
This protein can potentially be a great target for impairing fungal growth through the RNAi mechanism of V. dahliae [2].
We designed this part to test if our bacteria could produce dsRNA that targets this protein.
References
[1]Bertholet, A. M., Chouchani, E. T., Kazak, L., Angelin, A., Fedorenko, A., Long, J. Z., Vidoni, S., Garrity, R., Cho, J., Terada, N., Wallace, D. C., Spiegelman, B. M., & Kirichok, Y. (2019). H+ transport is an integral function of the mitochondrial ADP/ATP carrier. In Nature (Vol. 571, Issue 7766, pp. 515–520). Springer Science and Business Media LLC. https://doi.org/10.1038/s41586-019-1400-3
[2] Su, X., Rehman, L., Guo, H., Li, X., Zhang, R., & Cheng, H. (2017). AAC as a Potential Target Gene to Control Verticillium dahliae. Genes, 8(1), 25. doi:10.3390/genes8010025
Sequence and Features
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21INCOMPATIBLE WITH RFC[21]Illegal XhoI site found at 98
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]