Difference between revisions of "Part:BBa K5241009"

 
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In brewing yeast, the GAL1 promoter, tyrosine as the target gene, and the CYC1 terminator are used to express the tyrosine synthesis pathway for the production of melanin in yeast.
 
In brewing yeast, the GAL1 promoter, tyrosine as the target gene, and the CYC1 terminator are used to express the tyrosine synthesis pathway for the production of melanin in yeast.
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Gene Circuit Diagram:
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<figure><img style="width: 65%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/9/bba-k5241009-img01.png"></figure>
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</html>
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Result:
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<html>
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<figure><img style="width: 65%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/9/bba-k5241009-img02.png"></figure>
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</html>
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We successfully expressed melanin in Saccharomyces cerevisiae and used it for ionizing radiation experiments.
  
 
<!-- Add more about the biology of this part here
 
<!-- Add more about the biology of this part here

Revision as of 08:05, 27 September 2024


GAL1-Tyrosinase(BM)-GAL1

In brewing yeast, the GAL1 promoter, tyrosine as the target gene, and the CYC1 terminator are used to express the tyrosine synthesis pathway for the production of melanin in yeast.

Gene Circuit Diagram:

Result:

We successfully expressed melanin in Saccharomyces cerevisiae and used it for ionizing radiation experiments.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 3371
    Illegal SpeI site found at 1073
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 3371
    Illegal BglII site found at 2217
    Illegal BamHI site found at 3117
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 3371
    Illegal XbaI site found at 1769
    Illegal SpeI site found at 1073
    Illegal NgoMIV site found at 10303
    Illegal AgeI site found at 382
    Illegal AgeI site found at 1844
    Illegal AgeI site found at 2604
    Illegal AgeI site found at 9917
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 5245
    Illegal SapI site found at 4162