Difference between revisions of "Part:BBa K5241003"
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Description:Tyrosinase that converts tyrosine to dopamine and dopamine to dopaquinone.
 
Description:Tyrosinase that converts tyrosine to dopamine and dopamine to dopaquinone.
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Gene Circuit Diagram:
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<figure><img style="width: 65%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/3/bba-k5241003-img01.png"></figure>
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Source:Priestia megaterium or Bacillus megaterium
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Result:Construct a brewing yeast vector driven by the GAL1 promoter, using Tyrosinase(BM) as the target gene. By adding an inducer to activate GAL1, the expression of the tyrosinase gene is induced, catalyzing the oxidation of tyrosine into dopamine, which is further oxidized and polymerized to form melanin.Melanin was successfully expressed in yeast.
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<html>
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<figure><img style="width: 65%; padding:28px;"src="https://static.igem.wiki/teams/5241/wiki/parts-static/3/bba-k5241003-img02.png"></figure>
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>
 
<partinfo>BBa_K5241003 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5241003 SequenceAndFeatures</partinfo>
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Construct a brewing yeast vector driven by the GAL1 promoter, using Tyrosinase(BM) as the target gene. By adding an inducer to activate GAL1, the expression of the tyrosinase gene is induced, catalyzing the oxidation of tyrosine into dopamine, which is further oxidized and polymerized to form melanin.Melanin was successfully expressed in yeast.
 
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https://static.igem.wiki/teams/5241/wiki/parts-static/bba-k5241003-img01.png
 
  
  

Revision as of 06:19, 27 September 2024


Tyrosinase(BM)

Description:Tyrosinase that converts tyrosine to dopamine and dopamine to dopaquinone.

Gene Circuit Diagram:

Source:Priestia megaterium or Bacillus megaterium

Result:Construct a brewing yeast vector driven by the GAL1 promoter, using Tyrosinase(BM) as the target gene. By adding an inducer to activate GAL1, the expression of the tyrosinase gene is induced, catalyzing the oxidation of tyrosine into dopamine, which is further oxidized and polymerized to form melanin.Melanin was successfully expressed in yeast.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 382
  • 1000
    COMPATIBLE WITH RFC[1000]