Difference between revisions of "Part:BBa K5152010"
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We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes. | We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes. | ||
| − | We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the | + | We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the pUC19 vector (<partinfo>K5152011</partinfo>). |
We verified that these regions allow for successful and effective assembly. | We verified that these regions allow for successful and effective assembly. | ||
Revision as of 00:08, 27 September 2024
5' 20 nt overlapping region for pUC19 PstI site
We incorporated overlapping regions into each insert because we're using the HiFi assembly method for assembling all the insert parts in our project. The pUC19 vector was digested and linearized using PstI and EcoRI restriction enzymes.
We also designed a 20 nt overlapping region at the 3' end for the EcoRI site on the pUC19 vector (BBa_K5152011).
We verified that these regions allow for successful and effective assembly.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]