Difference between revisions of "Part:BBa K190015:Experience"

Revision as of 18:32, 21 October 2009

This experience page is provided so that any user may enter their experience using this part.
Please enter how you used this part and how it worked out.

Applications of BBa_K190015

BBa_J33201 (Available) E. coli chromosomal ars promoter with arsR repressor gene

BBa_J33203 (Available) E. coli ars promoter, arsR gene and lacZ

BBa_K190023 (Planning) E. coli ars promoter, with RBS site

BBa_K190033 (Planning) E. coli ars promoter, GVP gene cluster

User Reviews

UNIQ0e520d496c5221ad-partinfo-00000000-QINU

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iGEM Groningen 2009

The part (BBa_K190015) was used in our overall arsenic clean up E.coli to activate gas vesicle production upon arsenic detection. The promoter seemed to activate formation of vesicles without addition of arsenic in our tests. Either the ArsR regulator protein is produced in a too low amount to control unwanted expression (in witch case the gene for ArsR has to be upregulated), or the arsenic levels in our water are already sufficient for activation of expression.

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iGEM Groningen 2009

Relative promoter units were calculated from RFP fluorescence upon arsenite induction of the ArsR-promoter. The promoter had an induction of 2.26 (RPU) after induction for 3hrs with 100uM NaAsO2. This is an internal concentration between 0.8-1.2uM As (as seen in Figure 1).

Figure 1: RFP fluorescence increasing upon RFP expression under pArsR promoter (K190015)

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UNIQ0e520d496c5221ad-partinfo-00000004-QINU

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 ===Applications of BBa_K190015===
+[https://parts.igem.org/wiki/index.php/Part:BBa_J33201 BBa_J33201]   (Available)	E. coli chromosomal ars promoter with arsR repressor gene
+[https://parts.igem.org/wiki/index.php/Part:BBa_J33203 BBa_J33203]	(Available)	E. coli ars promoter, arsR gene and lacZ
+[https://parts.igem.org/wiki/index.php/Part:BBa_K190023 BBa_K190023]     (Planning)        E. coli ars promoter, with RBS site
+[https://parts.igem.org/wiki/index.php/Part:BBa_K190033 BBa_K190033]     (Planning)        E. coli ars promoter, GVP gene cluster
 ===User Reviews===