Difference between revisions of "Part:BBa K5115050"
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===Usage and Biology===
 
===Usage and Biology===
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In our design, MTA mainly works with  [https://parts.igem.org/Part:BBa_K5115000 BBa_K5115000(RcnR_C35L)]and  [https://parts.igem.org/Part:BBa_1151001 BBa_K1151001(hpn)]. They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.
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In our design, MTA mainly works with  [https://parts.igem.org/Part:BBa_K5115000 BBa_K5115000(RcnR_C35L)]and  [https://parts.igem.org/Part:BBa_K1151001 BBa_K1151001(hpn)]. They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.
  
 
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Revision as of 16:40, 26 September 2024


MTA, Metallothionein

contributed by Fudan iGEM 2023

Introduction

Metallothioneins (MTA) are intracellular, low molecular, low molecular weight, cysteine-rich proteins. Ubiquitous in eukaryotes, MTA have unique structural characteristics to give potent metal-binding and redox capabilities.[1] Closely binding with nickel ion, MTA can reduce the harm this metal may cause to the bacteria.

Usage and Biology

In our design, MTA mainly works with BBa_K5115000(RcnR_C35L)and BBa_K1151001(hpn). They can raise the absorptivity of nickel ion as well as reduce the harm nickel brings to our engineering bacteria.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 123
  • 1000
    COMPATIBLE WITH RFC[1000]


  1. ↑ Coyle, P., Philcox, J. C., Carey, L. C., & Rofe, A. M. (2002). Metallothionein: The multipurpose protein. Cellular and Molecular Life Sciences: CMLS, 59(4), 627–647.