Difference between revisions of "Part:BBa K5324001:Design"
Jacksonhall (Talk | contribs) (→References) |
Jacksonhall (Talk | contribs) (→Design Notes) |
||
| Line 7: | Line 7: | ||
===Design Notes=== | ===Design Notes=== | ||
| − | This sequence was codon optimized for expression in E.coli. Bsal and Bsml restriction cut sites. | + | This sequence was codon optimized for expression in E.coli. Bsal and Bsml restriction cut sites removed with 1 bp mutations. |
===Source=== | ===Source=== | ||
Revision as of 16:38, 26 September 2024
Destructin-1
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12INCOMPATIBLE WITH RFC[12]Illegal NotI site found at 55
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25INCOMPATIBLE WITH RFC[25]Illegal NgoMIV site found at 832
- 1000COMPATIBLE WITH RFC[1000]
Design Notes
This sequence was codon optimized for expression in E.coli. Bsal and Bsml restriction cut sites removed with 1 bp mutations.
Source
Genomic sequence obtained from GeneBank. Originated from P. destructans.
References
Bennett, R. J. (2015, April 15). Destructin-1 is a collagen-degrading endopeptidase ... Destructin-1 is a collagen-degrading endopeptidase secreted by Pseudogymnoascus destructans, the causative agent of white-nose syndrome. https://www.pnas.org/doi/full/10.1073/pnas.1507082112 U.S. National Library of Medicine. (n.d.). RecName: Full=subtilisin-like protease 2; AltName: Full=destructin-1;. National Center for Biotechnology Information. https://www.ncbi.nlm.nih.gov/protein/L8FSM5.1