Difference between revisions of "Part:BBa K5115036"

Line 6: Line 6:
 
__TOC__
 
__TOC__
 
===Introduction===
 
===Introduction===
This composite part is composed of hpn and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hpn is a protein which can bind with nickel ion, get details in  [https://parts.igem.org/Part:BBa_K1151001 BBa_K1151001(Histidine-rich metal-binding protein)]
+
This composite part is composed of hpn and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hpn is a protein which can bind with nickel ions, get details in  [https://parts.igem.org/Part:BBa_K1151001 BBa_K1151001(Histidine-rich metal-binding protein)]
  
 
===Usage and Biology===
 
===Usage and Biology===

Revision as of 15:30, 26 September 2024


ribozyme+RBS+Hpn+stem-loop

contributed by Fudan iGEM 2023

Introduction

This composite part is composed of hpn and improved ribozyme-assisted polycistronic co-expression system: pRAP. The hpn is a protein which can bind with nickel ions, get details in BBa_K1151001(Histidine-rich metal-binding protein)

Usage and Biology

The ribozyme-assisted polycistronic co-expression system can ensure that each cistron can initiate translation with comparable efficiency. For more information, please check part wiki of 2022 Fudan iGEM.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References