Difference between revisions of "Part:BBa K5317014"
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===Usage and Biology=== | ===Usage and Biology=== | ||
| − | + | =Cloning= | |
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| + | ===Theoretical Part Design=== | ||
| + | Placing the mRuby reporter gene behind ccpA allows for visualisation of expression of the protein of interest. | ||
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| + | ===Sequence and Features=== | ||
| + | <partinfo>BBa_K5317014 SequenceAndFeatures</partinfo> | ||
===References=== | ===References=== | ||
Revision as of 14:20, 26 September 2024
CcpA
The regulatory functions of CcpA are modulated by phosphorylation by serine/threonine kinases, which can affect its DNA-binding activity and thus its ability to regulate target genes. This phosphorylation-dependent mechanism enables S. aureus to adapt to different environmental conditions, thereby increasing its survivability and virulence (Liao et al., 2022). The CcpA inactivation impairs biofilm formation and restricts the incorporation of extracellular DNA (eDNA) into the biofilm matrix, while codY inactivation leads to a structured but robust biofilm bound to eDNA and intercellular adhesin polysaccharide (PIA) in S. aureus(Bulock et al., 2022).