Difference between revisions of "Part:BBa K5477009"

 
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<partinfo>BBa_K5477009 short</partinfo>
 
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The Lex6Op refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand.   
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The Lex6Op refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand (1) (2).   
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In our reporter module, the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα or ERRγ will bind to these operators that drive the expression of our reporter gene NanoLuc.
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<partinfo>BBa_K5477009 parameters</partinfo>
 
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===References===
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1. Shaw, W.M., et al., Engineering a Model Cell for Rational Tuning of GPCR Signaling. Cell, 2019. 177(3): p. 782-796 e27.
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2. Zhou, T., Liang, Z. & Marchisio, M.A. Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol. Sci Rep 12, 21791 (2022). https://doi.org/10.1038/s41598-022-26195-x

Revision as of 09:53, 26 September 2024


Lex6Op - six tandem copies of LexA operator

The Lex6Op refers to six tandem copies of the LexA operator. These operator sequences serve as binding sites for transcription factors that contain a LexA DNA-binding domain. This creates a platform for transcriptional activation of the reporter system upon presence of the ligand (1) (2).

In our reporter module, the Lex6Op is positioned upstream of the promoter (pLEU2), meaning the LexA fused with either the wildtype or mutant ERα or ERRγ will bind to these operators that drive the expression of our reporter gene NanoLuc.


Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


References

1. Shaw, W.M., et al., Engineering a Model Cell for Rational Tuning of GPCR Signaling. Cell, 2019. 177(3): p. 782-796 e27.

2. Zhou, T., Liang, Z. & Marchisio, M.A. Engineering a two-gene system to operate as a highly sensitive biosensor or a sharp switch upon induction with β-estradiol. Sci Rep 12, 21791 (2022). https://doi.org/10.1038/s41598-022-26195-x