Difference between revisions of "Part:BBa K257023:Experience"
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how you used this part and how it worked out. | how you used this part and how it worked out. | ||
| − | === | + | ===Caracterisation of BBa_K257023=== |
| − | + | Overnight cultures of BBa_K257023 were diluted 1/100 in LB or M9 medium supplemented with varing amont of FeNO3 and Na Citrate. Fluorescence and OD were assessed in a Tecan plate reader. Fluorescence divided by OD is ploted. | |
| − | + | [[Image:PFec-mRFP1-LB.JPG|Pfec caracterisation|500px]] | |
| + | [[Image:PFec-mRFP1-M9.JPG|Pfec caracterisation|500px]] | ||
| + | |||
| + | The low Fluo/OD at high FeNO3 concentration can be explained by the taxicity of FeNO3. Note that the best response to Na Citrate is obtained with 1mM FeNO3. | ||
===User Reviews=== | ===User Reviews=== | ||
Revision as of 18:08, 21 October 2009
This experience page is provided so that any user may enter their experience using this part.
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how you used this part and how it worked out.
Caracterisation of BBa_K257023
Overnight cultures of BBa_K257023 were diluted 1/100 in LB or M9 medium supplemented with varing amont of FeNO3 and Na Citrate. Fluorescence and OD were assessed in a Tecan plate reader. Fluorescence divided by OD is ploted.
The low Fluo/OD at high FeNO3 concentration can be explained by the taxicity of FeNO3. Note that the best response to Na Citrate is obtained with 1mM FeNO3.
User Reviews
UNIQf0229015805a4f92-partinfo-00000000-QINU UNIQf0229015805a4f92-partinfo-00000001-QINU