Difference between revisions of "Part:BBa K5461000"

 
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===Usage and Biology===
 
===Usage and Biology===
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This part is capable of expressing a fusion protein comprising CSGA-SpyTag with a GST protein tag. The GST tag facilitates protein purification, while CSGA has been demonstrated to tightly bind to cellulose and enhance its mechanical properties. Additionally, SpyTag can covalently bind to SpyCatcher. This architectural design allows the fusion protein to serve as a scaffold for modifying cellulose. In our project, we express SpyCatcher and the protein of interest (POI) as a fusion protein, which is then combined with cellulose, thereby endowing cellulose with the properties of various functional proteins.
 
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<span class='h3bb'>Sequence and Features</span>
 
<span class='h3bb'>Sequence and Features</span>

Revision as of 07:07, 26 September 2024


GST-csgA-spytag

CsgA is a biofilm protein that does not assemble within E. coli but can self-assemble into fibrous structures when expressed in vitro. This part is designed to express a protein with a GST purification tag and a SpyTag oligopeptide, under the control of the T7 promoter. We have expressed the fusion protein csgA-SpyTag with a GST tag within E. coli and successfully purified it using column chromatography. In our system, the csgA-SpyTag fusion protein serves as a linker between cellulose and the target protein.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 85