Difference between revisions of "Part:BBa K195625"

Latest revision as of 18:00, 21 October 2009

CII/PenI Damped Oscillator (II)

This device is produces damped oscillations between PenI and CII. The pRE can be activated by CII, which in turn produces PenI and GFP. The PenI then represses the promoter pPenI pPenI resulting in slower production of CII.

For testing this part, we use the CII/PenI Damped Oscillator With GFP Reporter (II) and use GFP to measure the oscillation.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
INCOMPATIBLE WITH RFC[21]
Illegal BglII site found at 516
23
COMPATIBLE WITH RFC[23]
25
INCOMPATIBLE WITH RFC[25]
Illegal AgeI site found at 334
1000
COMPATIBLE WITH RFC[1000]

@@ Line 1: / Line 1: @@
 __NOTOC__
 <partinfo>BBa_K195625 short</partinfo>
-This main part of the time delay device (II).
+This device is produces damped oscillations between PenI and CII. The [[Part:BBa_K116603|pRE]] can be activated by [[Part:BBa_K116602|CII]], which in turn produces [[Part:BBa_C0074|PenI]] and GFP. The PenI then represses the promoter pPenI [[Part:BBa_R0074|pPenI]] resulting in slower production of CII.
-The promoter pRE could be induced by BBa_K116602,the inducer CII, which results in the production of the TetR and green fluorescent protein. PenI from Bacillus licheniformis with LVA tail could repress the regulatory promoter BBa_R0074 pPenI, which results in the lower production of the inducer CII.
+For testing this part, we use the [[Part:BBa_K195623|CII/PenI Damped Oscillator With GFP Reporter (II)]] and use GFP to measure the oscillation.
 <!-- Add more about the biology of this part here