Difference between revisions of "Part:BBa I750016"
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<partinfo>BBa_I750016 parameters</partinfo>
 
<partinfo>BBa_I750016 parameters</partinfo>
 
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==Bouyancy Tests==
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==Electron Micrograph of Gas Vesicles in ''E.coli'' protoplasts==
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When <partinfo>BBa_K190033</partinfo>, the GVP cluster with an arsenic sensitive promoter (<partinfo>BBa_K190015</partinfo>) in front of it, is introduced in ''E.coli'' gas vesicles can be seen under the electron microscope (Figure 1).
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[[Image:GasvesiclesEM.JPG|thumb|500px|'''Fig. 1: Gas vesicles in E. coli protoplasts (<partinfo>BBa_K190033</partinfo>). The cells were treated with Lysozyme and SDS to create the protoplasts, uranyl acetate was used for staining. Magnification: 11500x. ([http://2009.igem.org/Team:Groningen Groningen 2009])]]

Revision as of 17:59, 21 October 2009

Gas Vesicle polycistonic gene

This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 334
    Illegal BamHI site found at 4139
    Illegal XhoI site found at 3827
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 785
    Illegal NgoMIV site found at 1187
    Illegal AgeI site found at 4106
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 2597
    Illegal SapI.rc site found at 3753


Bouyancy Tests

Electron Micrograph of Gas Vesicles in E.coli protoplasts

When BBa_K190033, the GVP cluster with an arsenic sensitive promoter (BBa_K190015) in front of it, is introduced in E.coli gas vesicles can be seen under the electron microscope (Figure 1).

Fig. 1: Gas vesicles in E. coli protoplasts (BBa_K190033). The cells were treated with Lysozyme and SDS to create the protoplasts, uranyl acetate was used for staining. Magnification: 11500x. ([http://2009.igem.org/Team:Groningen Groningen 2009])