Difference between revisions of "Part:BBa K5152005"

 
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<partinfo>BBa_K5152005 short</partinfo>
 
<partinfo>BBa_K5152005 short</partinfo>
  
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This part was used to create a cadmium-sensing biosensor for our project. Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colors.
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We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.
  
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===Usage and Biology===
 
===Usage and Biology===
  
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<span class='h3bb'><b>Sequence and Features</b></span>
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<span class='h3bb'>Sequence and Features</span>
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<partinfo>BBa_K5152005 SequenceAndFeatures</partinfo>
 
<partinfo>BBa_K5152005 SequenceAndFeatures</partinfo>
  

Revision as of 01:13, 26 September 2024

pCadA cadmium sensing chromoprotein reporter device

This part was used to create a cadmium-sensing biosensor for our project. Our goal is to develop heavy metal biosensors that don't require special techniques or expensive equipment, so we used chromoproteins for their visible colors.

We designed this part and ordered it from IDT gBlock. However, we consistently failed to obtain successful transformants for unknown reasons. Despite using the same assembly process as other constructs, which succeeded, this one did not. The cause remains unclear.

Usage and Biology

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 228
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]