Difference between revisions of "Part:BBa K5492721"

Revision as of 20:59, 25 September 2024

J1_aptamer_with_fluorophore

J1 ssDNA aptamer sequence designed to specifically bind to histamine. The sequence contains a fluorophore molecule at its 5' end (5' Cy5™). j1_reverse_compliment_strand is complementary to this sequence, which contains a quencher molecule (3'Iowa Black® RQ) on its 3' end, which absorbs the light emitted by fluorophore until the aptamer binds histamine.

Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 4: / Line 4: @@
 [https://parts.igem.org/Part:BBa_K5492711 J1 ssDNA aptamer] sequence designed to specifically bind to histamine.
-The sequence contains a fluorophore molecule at its 5' end.
+The sequence contains a fluorophore molecule at its 5' end (5' Cy5™).
-[https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequence, which contains a quencher molecule which absorbs the light emitted by fluorophore until the aptamer binds histamine.
+[https://parts.igem.org/Part:BBa_K5492731 j1_reverse_compliment_strand] is complementary to this sequence, which contains a quencher molecule (3'Iowa Black® RQ) on its 3' end, which absorbs the light emitted by fluorophore until the aptamer binds histamine.