Difference between revisions of "Part:BBa K5492712"
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<partinfo>BBa_K5492712 short</partinfo> | <partinfo>BBa_K5492712 short</partinfo> | ||
− | S1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492702 s1_aptamer_connector]. The connector sequence contains a 5' biotin tag that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection. | + | S1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492702 s1_aptamer_connector]. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection. |
===Usage and Biology=== | ===Usage and Biology=== | ||
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− | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
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+ | https://static.igem.wiki/teams/5492/registry/aptamer-beads-illustr-small.jpg | ||
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<partinfo>BBa_K5492712 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492712 SequenceAndFeatures</partinfo> | ||
Revision as of 20:28, 25 September 2024
S1_aptamer
S1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to s1_aptamer_connector. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.
Usage and Biology
Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]