Difference between revisions of "Part:BBa K5492711"

Revision as of 20:27, 25 September 2024

J1_aptamer

J1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to j1_y1_aptamer_connector. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.

Usage and Biology

Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.

Sequence and Features

Assembly Compatibility:

10
COMPATIBLE WITH RFC[10]
12
COMPATIBLE WITH RFC[12]
21
COMPATIBLE WITH RFC[21]
23
COMPATIBLE WITH RFC[23]
25
COMPATIBLE WITH RFC[25]
1000
COMPATIBLE WITH RFC[1000]

@@ Line 3: / Line 3: @@
 <partinfo>BBa_K5492711 short</partinfo>
-J1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492701 j1_y1_aptamer_connector]. The connector sequence contains a 5' biotin tag that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.
+J1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492701 j1_y1_aptamer_connector]. The connector sequence contains a 5' biotin tag (5'biosg) that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.
@@ Line 10: / Line 10: @@
 <!-- -->
-<span class='h3bb'>Sequence and Features</span>
+===<span class='h3bb'>Sequence and Features</span>===
+https://static.igem.wiki/teams/5492/registry/aptamer-beads-illustr-small.jpg
 <partinfo>BBa_K5492711 SequenceAndFeatures</partinfo>