Difference between revisions of "Part:BBa K5492710"
Line 3: | Line 3: | ||
<partinfo>BBa_K5492710 short</partinfo> | <partinfo>BBa_K5492710 short</partinfo> | ||
− | Y1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492701 j1_y1_aptamer_connector]. The connector sequence contains a 5' biotin tag that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection. | + | Y1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to [https://parts.igem.org/Part:BBa_K5492701 j1_y1_aptamer_connector]. The connector sequence contains a 5' biotin (5'biosg) tag that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection. |
Line 10: | Line 10: | ||
<!-- --> | <!-- --> | ||
− | <span class='h3bb'>Sequence and Features</span> | + | ===<span class='h3bb'>Sequence and Features</span>=== |
+ | |||
+ | https://static.igem.wiki/teams/5492/registry/aptamer-beads-illustr-small.jpg | ||
+ | |||
<partinfo>BBa_K5492710 SequenceAndFeatures</partinfo> | <partinfo>BBa_K5492710 SequenceAndFeatures</partinfo> | ||
Revision as of 20:26, 25 September 2024
Y1_aptamer
Y1 ssDNA aptamer sequence designed to specifically bind to histamine. The overhang of the 5' end of the sequence is complementary to j1_y1_aptamer_connector. The connector sequence contains a 5' biotin (5'biosg) tag that lets the sequence bind to an avidin-coated magnetic bead via avidin-biotin connection.
Usage and Biology
Aptamers are generally artificial ssDNA, RNA, or peptide oligomers which bind to specific target molecules. All ssDNA aptamers we utilise are proven to be able to bind specifically to histamine, thus preventing the binding of the molecule to a histamine receptor.
Sequence and Features
Assembly Compatibility:
- 10COMPATIBLE WITH RFC[10]
- 12COMPATIBLE WITH RFC[12]
- 21COMPATIBLE WITH RFC[21]
- 23COMPATIBLE WITH RFC[23]
- 25COMPATIBLE WITH RFC[25]
- 1000COMPATIBLE WITH RFC[1000]