Difference between revisions of "Part:BBa K5492403"
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Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1].
 
Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into [https://parts.igem.org/Part:BBa_K5492101 pETDUET-1].
 +
This part uses [https://parts.igem.org/Part:BBa_K5492100 BBa_K5492100] designed for the conservation of sequences during synthesis and PCR reactions.
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Adapter sequences from GenScript are added to the 5' and 3' end of the part.
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The primers for this sequence can be found here: [https://parts.igem.org/Part:BBa_K5492020 BBa_K5492020] [https://parts.igem.org/Part:BBa_K5492021 BBa_K5492021]
  
  

Revision as of 20:08, 25 September 2024


Optimised HNMT - pETDUET-1

Optimised coding sequence of human Histamine-N-Methyltransferase appropiated for insertion into pETDUET-1. This part uses BBa_K5492100 designed for the conservation of sequences during synthesis and PCR reactions. Adapter sequences from GenScript are added to the 5' and 3' end of the part. The primers for this sequence can be found here: BBa_K5492020 BBa_K5492021


Usage and Biology

Histamine-N-Methlytransferase (HNMT) takes part in the inactivation of histamine. It transfers a methyl group to histamine, and the resulting N-methyl histamine isn’t able to bind to histamine receptors, thus reducing the biological effect of the compound. Limiting the level of histamine effect can be an appropriate approach in cases of inflammation, where you can anticipate the histamine level increase. On our skin this includes some chronic diseases such as psoriasis, but more often would be useful in handling the acute increase of histamine level, such as a wasp bite. The decrease in oral histamine administration may also help to prevent some acute GI inflammation and smoothing the symptoms of some chronic bowel diseases.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal BglII site found at 1015
    Illegal BamHI site found at 123
    Illegal XhoI site found at 1064
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 129
    Illegal EcoRI site found at 235
    Illegal XbaI site found at 47
    Illegal NgoMIV site found at 1034
  • 1000
    COMPATIBLE WITH RFC[1000]